	US 12,439,868 B2
	Method of enhancing lipid production in algae and applications thereof
Gurumurthy Raja Krishna Kumar, Navimumbai (IN); Meenakshi Sundaram Chelliah, Jamnagar (IN); Meghna Rajvanshi, Mumbai (IN); G Venkata Subhash, Eleuru (IN); Dhairyarajsinh Rajendrasinh Gohil, Jamnagar (IN); Sridharan Govindachary, Montreal (CA); and Santanu Dasgupta, Asansol (IN)
Assigned to Reliance Industries Limited, Mumbai (IN)
Filed by Reliance Industries Limited, Maharashtra (IN)
Filed on Feb. 26, 2021, as Appl. No. 17/186,834.
Claims priority of application No. 202021008421 (IN), filed on Feb. 27, 2020.
Prior Publication US 2021/0267152 A1, Sep. 2, 2021
Int. Cl. A01G 33/00 (2006.01); A01H 1/00 (2006.01); A01H 3/02 (2006.01); A01H 13/00 (2006.01); A01N 3/00 (2006.01); C12N 1/12 (2006.01); C12P 7/649 (2022.01)

CPC A01H 1/101 (2021.01) [A01G 33/00 (2013.01); A01H 13/00 (2013.01); A01N 3/00 (2013.01); C12N 1/12 (2013.01); C12P 7/649 (2013.01)]

14 Claims

1. A method of enhancing lipid production in algae, said method comprising:

(a) culturing algae in a condition of nutrient repletion followed by nutrient depletion;

(b) subsequently maintaining said algae in a thin layer cultivation system at a culture depth of about 1 cm to 10 cm, wherein the algae is subjected to non-shear mixing in the thin layer cultivation system to provide equal exposure to sunlight and CO₂, wherein said algae is maintained in nutrient deplete conditions in said thin layer cultivation system, wherein the algae maintained in the thin layer cultivation system is concentrated algal culture having a cell density ranging from about 5 g/L to 100 g/L, and