| CPC C07K 14/7051 (2013.01) [A61K 40/11 (2025.01); A61K 40/22 (2025.01); A61K 40/32 (2025.01); A61K 40/418 (2025.01); C07K 14/4718 (2013.01); C07K 14/70503 (2013.01); C07K 14/7158 (2013.01); C12N 5/0636 (2013.01); C12N 9/22 (2013.01); C12N 9/96 (2013.01); C12N 15/113 (2013.01); C12N 15/907 (2013.01); A61K 2239/38 (2023.05); C12N 15/87 (2013.01); C12N 2310/20 (2017.05); C12N 2510/00 (2013.01)] |
| AS A RESULT OF REEXAMINATION, IT HAS BEEN DETERMINED THAT: |
| Claims 1-8 are cancelled. |
| New claims 9 and 10 are added and determined to be patentable. |
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administering to the human subject an effective amount of a pharmaceutical composition that comprises (i) a population of genetically modified human natural killer (NK) cells that comprise a genomic disruption in an endogenous cytokine inducible SH2-containing (CISH) gene that suppresses or eliminates expression of a cytokine inducible SH2-containing (CISH) protein encoded by said CISH gene; and (ii) a pharmaceutically acceptable carrier or excipient.
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[ 9. A method of treating cancer in a human subject, the method comprising:
administering to the human subject an effective amount of a pharmaceutical composition that comprises (i) a population of genetically modified human natural killer (NK) cells that comprise a genomic disruption in an endogenous cytokine inducible SH2-containing (CISH) gene that suppresses or eliminates expression of a cytokine inducible SH2-containing (CISH) protein encoded by said CISH gene, wherein said genomic disruption is a nucleotide insertion or deletion in exon 2 or exon 3 of said CISH gene; and (ii) a pharmaceutically acceptable carrier or excipient.]
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[ 10. A method of treating cancer in a human subject, the method comprising:
administering to the human subject an effective amount of a pharmaceutical composition that comprises (i) a population of genetically modified human natural killer (NK) cells that comprise a genomic disruption in an endogenous cytokine inducible SH2-containing (CISH) gene that suppresses or eliminates expression of a cytokine inducible SH2-containing (CISH) protein encoded by said CISH gene, wherein said genomic disruption is a nucleotide insertion or deletion in said CISH gene, and wherein said genomic disruption is generated using a guide RNA that binds a target sequence within exon 2 or exon 3 of said CISH gene.; and (ii) a pharmaceutically acceptable carrier or excipient.]
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