US 12,111,455 B2
Detecting movements of a sample with respect to an objective
Roman Schmidt, Göttingen (DE); and Lars Kastrup, Göttingen (DE)
Assigned to Abberior Instruments GmbH, Goettingen (DE)
Filed by Abberior Instruments GmbH, Göttingen (DE)
Filed on Sep. 23, 2021, as Appl. No. 17/482,752.
Application 17/482,752 is a continuation in part of application No. PCT/EP2020/059415, filed on Apr. 2, 2020.
Claims priority of application No. 10 2019 108 696.5 (DE), filed on Apr. 3, 2019.
Prior Publication US 2022/0011559 A1, Jan. 13, 2022
Int. Cl. G02B 21/00 (2006.01); G02B 5/30 (2006.01); G02B 21/02 (2006.01); G02B 21/36 (2006.01); G02B 27/28 (2006.01)
CPC G02B 21/008 (2013.01) [G02B 5/3083 (2013.01); G02B 21/0032 (2013.01); G02B 21/02 (2013.01); G02B 21/365 (2013.01); G02B 27/283 (2013.01)] 53 Claims
OG exemplary drawing
 
1. An apparatus for detecting movements of a sample with respect to an objective, the apparatus comprising
imaging optics which
include the objective,
have an image plane and
are configured to image light from at least one reference object that is connected to the sample arranged in front of the objective into reference object images in the image plane,
a camera which is arranged in the image plane of the imaging optics and which is configured to record the reference object images at consecutive points in time, and
an optical device arranged between the objective and the camera,
wherein the optical device is arranged in a plane that is Fourier-conjugated with respect to the image plane and wherein the optical device is configured to mask out low spatial frequencies of reference object images which the imaging optics image into the image plane, and wherein the illumination light source is configured and arranged to focus the illumination light on an optical axis of the objective into a back focal plane of the objective to wide-field illuminate the sample arranged in front of the objective with the illumination light.