US 12,111,313 B2
Chelator-coated field effect transistor and devices and methods using same
Roger D. Kornberg, Atherton, CA (US)
Assigned to Autonomous Medical Devices Inc., Santa Ana, CA (US)
Appl. No. 17/264,281
Filed by SENSOR-KINESIS CORPORATION, Los Angeles, CA (US)
PCT Filed Aug. 14, 2019, PCT No. PCT/US2019/046568
§ 371(c)(1), (2) Date Jan. 28, 2021,
PCT Pub. No. WO2020/037078, PCT Pub. Date Feb. 20, 2020.
Claims priority of provisional application 62/718,632, filed on Aug. 14, 2018.
Prior Publication US 2021/0293798 A1, Sep. 23, 2021
Int. Cl. G01N 33/543 (2006.01); G01N 27/414 (2006.01)
CPC G01N 33/54373 (2013.01) [G01N 27/4145 (2013.01); G01N 27/4146 (2013.01); G01N 33/5432 (2013.01)] 22 Claims
OG exemplary drawing
 
1. A method for the detection of a target marker in a solution including charged ions and unrelated ions comprising:
providing a liposome in solution containing metal ions, the liposome including a detection antibody configured to bind a target marker;
providing a field-effect transistor with a source-drain channel comprising a surface functionalized with both a capture antibody to selectively bind to the target marker and a metal ion chelator or metal ion derivatized chelator;
selectively binding the target marker to the detection antibody included with the liposome;
selectively binding the target marker to the capture antibody to dispose the target marker and bound liposome to the source-drain channel of the field-effect transistor;
releasing the metal ions from the liposome;
selectively binding the metal ion chelator or metal ion derivatized chelator disposed on the functionalized surface of the source-drain channel with the metal ions released from the liposome; and
causing a change in current in the field-effect transistor as a result of the selective binding of the metal ion chelator or metal ion derivatized chelator with the metal ions released from the liposome without interference of the detection of the metal ions by screening oppositely charged ions and unrelated ions in the solution; and
wherein the change in current is indicative of detection of the target marker.