US 12,111,302 B2
Diagnostic assay for rumen undegraded protein detection
Keith E. Bruinsma, Brookings, SD (US); Donald L. Endres, Brookings, SD (US); Andrew James Manning, Sioux Falls, SD (US); and Kurt M. Swenson, Beulah, ND (US)
Assigned to Novita Nutrition, LLC, Brookings, SD (US)
Appl. No. 17/285,460
Filed by Novita Nutrition, LLC, Brookings, SD (US)
PCT Filed Oct. 10, 2019, PCT No. PCT/US2019/055710
§ 371(c)(1), (2) Date Apr. 14, 2021,
PCT Pub. No. WO2020/081371, PCT Pub. Date Apr. 23, 2020.
Claims priority of provisional application 62/746,692, filed on Oct. 17, 2018.
Prior Publication US 2021/0341446 A1, Nov. 4, 2021
Int. Cl. G01N 33/02 (2006.01); G01N 27/447 (2006.01); G01N 33/68 (2006.01)
CPC G01N 33/02 (2013.01) [G01N 27/447 (2013.01); G01N 27/44726 (2013.01); G01N 33/6803 (2013.01); Y10T 436/25375 (2015.01)] 21 Claims
 
1. A method for testing a food product for rumen undegraded protein, the method comprising:
extracting a total protein content from an assay sample of the food product to provide an extraction sample, wherein the total protein content of the extraction sample comprises a rumen undegraded protein content;
conducting gel electrophoresis on the extraction sample applied to a first gel and a second gel to separate the rumen undegraded protein content from the total protein content, thereby providing a residual total protein content;
staining the first gel after separating the rumen undegraded protein content from the total protein content, wherein staining the first gel comprises applying a stain capable of staining glycosylated proteins in a polyacrylamide gel such that the stained glycosylated proteins correspond to the separated rumen undegraded protein content;
staining the second gel after separating the rumen undegraded protein content from the total protein content, wherein staining the second gel comprises applying a stain capable of staining the total protein content in the extraction sample including both the separated rumen undegraded protein content and the residual total protein content in a polyacrylamide gel;
and conducting an intensity analysis of the stained separated rumen undegraded protein content in the first gel and the stained total protein content in the second gel to determine a percentage of rumen undegraded protein in the total protein content of the food product.