US 12,111,239 B2
Methods and kits for isolating whole cells from frozen tissues for cell fingerprinting
Tattym E. Shaiken, Houston, TX (US)
Assigned to PERI-NUC LABS, LLC, Houston, TX (US)
Filed by Peri-Nuc Labs LLC, Houston, TX (US)
Filed on May 5, 2021, as Appl. No. 17/308,393.
Claims priority of provisional application 63/020,770, filed on May 6, 2020.
Prior Publication US 2021/0348993 A1, Nov. 11, 2021
Int. Cl. G01N 1/28 (2006.01); B01L 3/02 (2006.01); G01N 1/40 (2006.01)
CPC G01N 1/4055 (2013.01) [B01L 3/021 (2013.01); G01N 1/286 (2013.01); G01N 1/4077 (2013.01); G01N 2001/2873 (2013.01)] 12 Claims
OG exemplary drawing
 
1. A method of isolating whole cells from a frozen tissue sample comprising:
(a) providing a frozen tissue sample, wherein the frozen tissue sample contains whole cells and stroma material;
(b) cutting the frozen tissue sample into two or more tissue pieces using a tissue sample cutter, wherein the tissue sample cutter has a plurality of cutting blades;
(c) forming an extraction mixture by incubating the two or more tissue pieces in a volume of tissue sample solution (TSS) for an incubation period;
(d) forming a whole cell sample by pipetting a first portion of the extraction mixture from the tissue sample solution into an extraction pipette, and then
(e) pipetting a second portion of the extraction mixture from the extraction pipette into a washing solution; and
(f) collecting isolated whole cells from the whole cell sample by flowing the extraction mixture through a first separation funnel into a collection vessel.