US 12,110,556 B2
Kit for assessing the risk of complications in patients with systemic inflammatory response syndrome (SIRS)
Elisabeth Cerrato, Lyons (FR); Benjamin Delwarde, Lyons (FR); Guillaume Monneret, Lyons (FR); Estelle Peronnet, Lyons (FR); Julien Textoris, Villeurbanne (FR); and Fabienne Venet, Lyons (FR)
Assigned to BIOMERIEUX, Marcy-l'Etoile (FR); HOSPICES CIVILS DE LYON, Lyons (FR); and UNIVERSITE CLAUDE BERNARD, Lyons (FR)
Filed by BIOMERIEUX, Marcy-l'etoile (FR); HOSPICES CIVILS DE LYON, Lyons (FR); and UNIVERSITE CLAUDE BERNARD LYON 1, Villeurbanne (FR)
Filed on Apr. 26, 2022, as Appl. No. 17/729,115.
Application 17/729,115 is a division of application No. 15/780,573, granted, now 11,339,438, previously published as PCT/FR2016/053164, filed on Dec. 1, 2016.
Claims priority of application No. 15 61671 (FR), filed on Dec. 1, 2015.
Prior Publication US 2022/0259661 A1, Aug. 18, 2022
Int. Cl. C12Q 1/6883 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/686 (2018.01)
CPC C12Q 1/6883 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/686 (2013.01); C12Q 2600/112 (2013.01); C12Q 2600/118 (2013.01); C12Q 2600/158 (2013.01)] 6 Claims
 
1. A kit for the in vitro measurement of a quantity of at least one transcript of the IL7R gene in a biological blood sample, comprising:
specific reagents for measuring the quantity of said at least one transcript of the IL7R gene in said biological blood sample, said at least one transcript comprising the transcript IL7R-001 of SEQ ID NO: 2, or a variant of the transcript IL7R-001 of SEQ ID NO: 2 having at least 99% identity with said sequence, including the following amplification primer pair: forward primer with SEQ ID NO: 12 and reverse primer with SEQ ID NO: 13 and a probe with SEQ ID NO: 14, said probe being either tagged with a marker, or immobilized on a solid support; and
a blood control sample that is a blood sample that is calibrated and contains the quantity of said at least one transcript of the IL7R gene that corresponds to the mean quantity measured in a pool of reference blood samples from human patients who are in a state of septic shock when the reference blood samples are taken, or who were in a state of septic shock within 72 hours after the reference blood samples were taken, and who were known to have survived, and/or a blood control sample that is a blood sample that is calibrated and contains the quantity of said at least one transcript of the IL7R gene that corresponds to the mean quantity measured in a pool of reference blood samples from human patients who are in a state of septic shock when the reference blood samples are taken, or who were in a state of septic shock within 72 hours after the reference blood samples were taken, and who were known not to have survived.