Methods for simultaneous amplification of target loci
Matthew Rabinowitz, San Francisco, CA (US); Matthew Micah Hill, Belmont, CA (US); Bernhard Zimmermann, Manteca, CA (US); Johan Baner, San Francisco, CA (US); George Gemelos, Portland, OR (US); Milena Banjevic, Los Altos Hills, CA (US); Allison Ryan, Belmont, CA (US); Styrmir Sigurjonsson, San Jose, CA (US); and Zachary Demko, San Francisco, CA (US)
Assigned to Natera, Inc., San Carlos, CA (US)
Filed by Natera, Inc., San Carlos, CA (US)
Filed on Apr. 23, 2020, as Appl. No. 16/856,924.
Application 16/856,924 is a continuation of application No. 16/743,724, filed on Jan. 15, 2020, granted, now 10,731,220.
Application 16/743,724 is a continuation of application No. 16/399,268, filed on Apr. 30, 2019, granted, now 10,538,814.
Application 16/399,268 is a continuation of application No. 16/140,298, filed on Sep. 24, 2018, abandoned.
Application 16/140,298 is a continuation of application No. 14/918,544, filed on Oct. 20, 2015, granted, now 10,316,362, issued on Jun. 11, 2019.
Application 14/918,544 is a continuation in part of application No. 14/877,925, filed on Oct. 7, 2015, abandoned.
Application 14/918,544 is a continuation in part of application No. 14/692,703, filed on Apr. 21, 2015, granted, now 10,179,937, issued on Jan. 15, 2019.
Application 14/918,544 is a continuation in part of application No. 14/538,982, filed on Nov. 24, 2014, granted, now 9,677,118, issued on Jun. 13, 2017.
Application 14/877,925 is a continuation in part of application No. 14/225,356, filed on Mar. 25, 2014, abandoned.
Application 14/877,925 is a continuation in part of application No. 13/780,022, filed on Feb. 28, 2013, abandoned.
Application 14/877,925 is a continuation in part of application No. 13/683,604, filed on Nov. 21, 2012, abandoned.
Application 14/225,356 is a continuation of application No. PCT/US2012/058578, filed on Oct. 3, 2012.
Application 13/780,022 is a continuation in part of application No. 13/683,604, filed on Nov. 21, 2012, abandoned.
Application 13/780,022 is a continuation in part of application No. PCT/US2012/058578, filed on Oct. 3, 2012.
Application 13/780,022 is a continuation in part of application No. 13/335,043, filed on Dec. 22, 2011, granted, now 10,113,196, issued on Oct. 30, 2018.
Application 13/780,022 is a continuation in part of application No. 13/300,235, filed on Nov. 18, 2011, granted, now 10,017,812, issued on Jul. 10, 2018.
Application 13/780,022 is a continuation in part of application No. 13/110,685, filed on May 18, 2011, granted, now 8,825,412, issued on Sep. 2, 2014.
Application 13/683,604 is a continuation in part of application No. 13/300,235, filed on Nov. 18, 2011, granted, now 10,017,812, issued on Jul. 10, 2018.
Application 13/683,604 is a continuation in part of application No. 13/110,685, filed on May 18, 2011, granted, now 8,825,412, issued on Sep. 2, 2014.
Application PCT/US2012/058578 is a continuation in part of application No. 13/300,235, filed on Nov. 18, 2011, granted, now 10,017,812, issued on Jul. 10, 2018.
Claims priority of provisional application 62/148,173, filed on Apr. 15, 2015.
Claims priority of provisional application 62/147,377, filed on Apr. 14, 2015.
Claims priority of provisional application 62/146,188, filed on Apr. 10, 2015.
Claims priority of provisional application 62/066,514, filed on Oct. 21, 2014.
Claims priority of provisional application 61/994,791, filed on May 16, 2014.
Claims priority of provisional application 61/987,407, filed on May 1, 2014.
Claims priority of provisional application 61/982,245, filed on Apr. 21, 2014.
Claims priority of provisional application 61/683,331, filed on Aug. 15, 2012.
Claims priority of provisional application 61/675,020, filed on Jul. 24, 2012.
Claims priority of provisional application 61/634,431, filed on Feb. 29, 2012.
Claims priority of provisional application 61/542,508, filed on Oct. 3, 2011.
Claims priority of provisional application 61/571,248, filed on Jun. 23, 2011.
Claims priority of provisional application 61/516,996, filed on Apr. 12, 2011.
Claims priority of provisional application 61/448,547, filed on Mar. 2, 2011.
Claims priority of provisional application 61/462,972, filed on Feb. 9, 2011.
Claims priority of provisional application 61/426,208, filed on Dec. 22, 2010.
Claims priority of provisional application 61/398,159, filed on Jun. 21, 2010.
Claims priority of provisional application 61/395,850, filed on May 18, 2010.
Prior Publication US 2020/0318191 A1, Oct. 8, 2020
1. A method for preparing a biological sample containing DNA from a first individual and a second individual, comprising:
isolating cell-free DNA from the biological sample, wherein the isolated cell-free DNA comprises the DNA from the first individual and DNA from the second individual;
amplifying the isolated cell-free DNA at a plurality of polymorphic loci on one or more chromosomes expected to be disomic, wherein the plurality of polymorphic loci comprises between 200 and 1,000 polymorphic loci, wherein the amplifying comprises amplifying the between 200 and 1,000 polymorphic loci from the cell-free DNA isolated from the biological sample using between 200 and 1,000 distinct primer pairs configure to target the between 200 and 1,000 polymorphic loci in a single reaction volume; and
preparing the isolated cell-free DNA amplified at the polymorphic loci for sequencing.