US 12,110,549 B2
Methods and systems for processing polynucleotides
Phillip Belgrader, Livermore, CA (US); Zachary Bent, Pleasanton, CA (US); Rajiv Bharadwaj, Pleasanton, CA (US); Vijay Kumar Sreenivasa Gopalan, Pleasanton, CA (US); Josephine Harada, San Francisco, CA (US); Christopher Hindson, Livermore, CA (US); Mohammad Rahimi Lenji, Pleasanton, CA (US); Michael Ybarra Lucero, South San Francisco, CA (US); Geoffrey McDermott, Livermore, CA (US); Elliott Meer, Pleasanton, CA (US); Tarjei Sigurd Mikkelsen, Dublin, CA (US); Christopher Joachim O'Keeffe, Pleasanton, CA (US); Katherine Pfeiffer, Berkeley, CA (US); Andrew D. Price, Hayward, CA (US); Paul Ryvkin, San Jose, CA (US); Serge Saxonov, Oakland, CA (US); John R. Stuelpnagel, Santa Barbara, CA (US); Jessica Michele Terry, Pleasanton, CA (US); Tobias Daniel Wheeler, Alameda, CA (US); Indira Wu, San Carlos, CA (US); Solongo Batjargal Ziraldo, San Ramon, CA (US); Stephane Claude Boutet, Burlingame, CA (US); Sarah Taylor, Pleasanton, CA (US); and Niranjan Srinivas, Dublin, CA (US)
Assigned to 10X GENOMICS, INC., Pleasanton, CA (US)
Filed by 10x Genomics, Inc., Pleasanton, CA (US)
Filed on Aug. 24, 2022, as Appl. No. 17/822,117.
Application 17/822,117 is a continuation of application No. 17/473,760, filed on Sep. 13, 2021.
Application 17/473,760 is a continuation of application No. 17/084,481, filed on Oct. 29, 2020, granted, now 11,180,805, issued on Nov. 23, 2021.
Application 17/084,481 is a continuation of application No. 16/443,638, filed on Jun. 17, 2019, granted, now 10,858,702, issued on Dec. 8, 2020.
Application 16/443,638 is a continuation of application No. 16/439,675, filed on Jun. 12, 2019, granted, now 10,815,525, issued on Oct. 27, 2020.
Application 16/439,675 is a continuation in part of application No. 15/933,299, filed on Mar. 22, 2018, granted, now 10,480,029, issued on Nov. 19, 2019.
Application 15/933,299 is a continuation of application No. 15/720,085, filed on Sep. 29, 2017, granted, now 10,011,872, issued on Jul. 3, 2018.
Application 16/439,675 is a continuation in part of application No. PCT/US2017/068320, filed on Dec. 22, 2017.
Application PCT/US2017/068320 is a continuation in part of application No. 15/720,085, filed on Sep. 29, 2017, granted, now 10,011,872, issued on Jul. 3, 2018.
Application 16/439,675 is a continuation in part of application No. PCT/US2018/064600, filed on Dec. 7, 2018.
Application PCT/US2018/064600 is a continuation in part of application No. 16/107,685, filed on Aug. 21, 2018, abandoned.
Application 16/439,675 is a continuation in part of application No. 16/107,685, filed on Aug. 21, 2018, abandoned.
Claims priority of provisional application 62/438,341, filed on Dec. 22, 2016.
Claims priority of provisional application 62/596,557, filed on Dec. 8, 2017.
Claims priority of provisional application 62/723,960, filed on Aug. 28, 2018.
Prior Publication US 2023/0203577 A1, Jun. 29, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/683 (2018.01); C12Q 1/6874 (2018.01)
CPC C12Q 1/6874 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/683 (2013.01); C12Q 2525/191 (2013.01); C12Q 2537/143 (2013.01); C12Q 2563/179 (2013.01)] 29 Claims
 
1. A method, comprising:
(a) providing a partition comprising:
(i) a labeled cell or a labeled organelle, wherein the labeled cell or the labeled organelle comprises a surface labeling agent, wherein the surface labeling agent is a small molecule attached to a reporter nucleic acid barcode molecule comprising a reporter barcode sequence, wherein the small molecule comprises a molecular weight of no greater than 1,000 Daltons (Da); and
(ii) a partition nucleic acid barcode molecule comprising a partition barcode sequence; and
(b) using the reporter nucleic acid barcode molecule and the partition nucleic acid barcode molecule to generate a barcoded nucleic acid molecule comprising (1) the reporter barcode sequence or a reverse complement thereof and (2) the partition barcode sequence or a reverse complement thereof.