US 12,110,546 B2
Tertiary amine substituted coumarin compounds and uses as fluorescent labels
Nikolai Nikolaevich Romanov, Cambridge (GB); Patrick McCauley, Cambridge (GB); and Niall Hynes, Cambridge (GB)
Assigned to Illumina Cambridge Limited, Cambridge (GB)
Filed by Illumina Cambridge Limited, Cambridge (GB)
Filed on Feb. 28, 2020, as Appl. No. 16/805,125.
Claims priority of provisional application 62/812,732, filed on Mar. 1, 2019.
Prior Publication US 2020/0277670 A1, Sep. 3, 2020
Int. Cl. C12Q 1/6869 (2018.01); C07D 405/04 (2006.01); C07D 413/04 (2006.01); C07D 417/04 (2006.01); C07H 19/06 (2006.01); C07H 19/16 (2006.01)
CPC C12Q 1/6869 (2013.01) [C07D 405/04 (2013.01); C07D 413/04 (2013.01); C07D 417/04 (2013.01); C07H 19/06 (2013.01); C07H 19/16 (2013.01)] 23 Claims
 
1. A nucleotide defined by the formula:

OG Complex Work Unit Chemistry
wherein B is a nucleobase;
OR′ is a triphosphate;
R″ is a 3′ blocking group;
R′″ is H;
L is a linker moiety; and
Dye comprises:

OG Complex Work Unit Chemistry