	US 12,110,540 B2
	Compositions and methods for detecting toxigenic Clostridium difficile
Patrick Lynn Peterson, San Diego, CA (US)
Assigned to GEN-PROBE INCORPORATED, San Diego, CA (US)
Appl. No. 16/769,229
Filed by Gen-Probe Incorporated
PCT Filed Dec. 13, 2018, PCT No. PCT/US2018/065470 § 371(c)(1), (2) Date Jun. 2, 2020, PCT Pub. No. WO2019/118735, PCT Pub. Date Jun. 20, 2019.
Claims priority of provisional application 62/599,494, filed on Dec. 15, 2017.
Prior Publication US 2020/0318171 A1, Oct. 8, 2020
Int. Cl. C12Q 1/689 (2018.01); C12Q 1/6827 (2018.01)

CPC C12Q 1/6827 (2013.01) [C12Q 1/689 (2013.01); C12Q 2537/143 (2013.01); C12Q 2561/109 (2013.01); C12Q 2565/101 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/166 (2013.01)]

12 Claims

1. A composition or kit comprising:

a detection oligomer comprising the sequence set forth in positions 7-18 of SEQ ID NO: 305, wherein the detection oligomer further comprises sufficient additional sequence to specifically hybridize to a C. difficile tcdB nucleic acid, and wherein the detection oligomer is non-extendable; and

at least one secondary detection oligomer, comprising a secondary detection oligomer that is configured to interact with a fragment of the detection oligomer, comprises at least two labels, and comprises the sequence of SEQ ID NO: 12.