	US 12,110,527 B2
	Production of symmetrical bolaform sophorosides
Sophie Roelants, Melle (BE); Lisa Van Renterghem, Evergem (BE); Wim Soetaert, Lievegem (BE); and Jelle Remmery, Ghent (BE)
Assigned to UNIVERSITEIT GENT, Ghent (BE); and AMPHISTAR, Lievegem (BE)
Appl. No. 17/292,092
Filed by UNIVERSITEIT GENT, Ghent (BE); and AMPHISTAR, Lievegem (BE)
PCT Filed Nov. 21, 2019, PCT No. PCT/EP2019/082063 § 371(c)(1), (2) Date May 7, 2021, PCT Pub. No. WO2020/104582, PCT Pub. Date May 28, 2020.
Claims priority of application No. 18207793 (EP), filed on Nov. 22, 2018.
Prior Publication US 2022/0033869 A1, Feb. 3, 2022
Int. Cl. C12P 19/44 (2006.01); C12N 9/02 (2006.01); C12N 9/04 (2006.01); C12N 15/01 (2006.01); C12P 1/02 (2006.01); C12P 7/6436 (2022.01)

CPC C12P 19/44 (2013.01) [C12N 9/0006 (2013.01); C12N 9/0071 (2013.01); C12N 15/01 (2013.01); C12Y 101/0302 (2013.01); C12Y 114/14001 (2013.01)]

13 Claims

1. A method to produce fully symmetrical bola sophorosides which are free of contaminating fatty acid-containing sophorolipids comprising feeding a mutated fungal strain with a fatty alcohol having an aliphatic tail chain length of at least 6 carbons,

wherein said fungal strain has a dysfunctional CYP52M1 cytochrome P450 monooxygenase and a dysfunctional FAO1 fatty alcohol oxidase, and

wherein said fungal strain is Starmerella (Candida) bombicola or a sophorolipid-producing strain of the Starmerella clade.