US 12,110,525 B2
Method for enzymatic preparation of R-3 aminobutyric acid
Chuanmin Sun, Shanghai (CN); Zhanbing Cheng, Shanghai (CN); Jianghua Jiao, Shanghai (CN); Shaonan Ding, Shanghai (CN); and Zhenhua Tian, Shanghai (CN)
Assigned to ABIOCHEM BIOTECHNOLOGY (GROUP) CO., LTD., Shanghai (CN)
Filed by ABIOCHEM BIOTECHNOLOGY CO., LTD., Shanghai (CN)
Filed on Dec. 1, 2021, as Appl. No. 17/539,414.
Application 17/539,414 is a division of application No. 16/652,312, granted, now 11,220,701, previously published as PCT/CN2018/092010, filed on Jun. 20, 2018.
Claims priority of application No. 201710906645.5 (CN), filed on Sep. 29, 2017.
Prior Publication US 2022/0090152 A1, Mar. 24, 2022
Int. Cl. C12N 9/88 (2006.01); C12P 13/04 (2006.01)
CPC C12P 13/04 (2013.01) [C12N 9/88 (2013.01); C12Y 403/01001 (2013.01)] 7 Claims
 
1. An R-3-aminobutyric acid production strain expressing a polypeptide, wherein the polypeptide is an exogenous aspartase derived from E. coli and is used to catalyze a stereoisomeric catalytic reaction as shown below:

OG Complex Work Unit Chemistry
wherein the exogenous aspartase is a mutant having 90% sequence identity to the amino acid sequence of SEQ ID NO: 5, possessing two to four amino acid mutations in the amino acid sequence corresponding to the wild type aspartase shown in SEQ ID NO: 5; or
wherein the exogenous aspartase is a mutant having an amino acid mutation selected from the group consisting of threonine (T) at position 204, methionine (M) at position 338, Lysine (K) at position 341, asparagine (N) at position 343, and a combination thereof.