	US 12,110,524 B2
	Generation of acyl alcohols
Kevin A. Jarrell, Lincoln, MA (US)
Assigned to Modular Genetics, Inc., Lincoln, MA (US)
Filed by Modular Genetics, Inc., Woburn, MA (US)
Filed on May 20, 2022, as Appl. No. 17/750,276.
Application 17/750,276 is a division of application No. 15/744,574, granted, now 11,371,066, previously published as PCT/US2016/042157, filed on Jul. 13, 2016.
Claims priority of provisional application 62/191,571, filed on Jul. 13, 2015.
Prior Publication US 2023/0039639 A1, Feb. 9, 2023
Int. Cl. C12N 9/90 (2006.01); C12P 7/6418 (2022.01); C12P 13/00 (2006.01); C12P 13/04 (2006.01)

CPC C12P 13/001 (2013.01) [C12N 9/90 (2013.01); C12P 7/6418 (2013.01); C12P 13/04 (2013.01); C12Y 501/01011 (2013.01)]

15 Claims

1. An engineered microbial cell comprising one or more polypeptides collectively comprising:

a fatty acid linkage domain comprising a condensation domain of a SrfA subunit of Bacillus subtilis's surfactin synthetase, a single peptide synthetase domain comprising an adenylation domain of a glycine module of Bacillus brevis's gramicidin peptide synthetase, and one or more reductase polypeptides comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 1,

which fatty acid linkage domain is covalently linked to the single peptide synthetase domain,

which fatty acid linkage domain and peptide synthetase domain collectively can produce an acyl amino acid,

and which one or more reductase polypeptides are collectively capable of reducing the acyl amino acid to an acyl amino alcohol.