	US 12,110,511 B2
	Methods and culture media for production, quantification and isolation of bacteriophages
Lotta-Riina Sundberg, Jyväskylä (FI); Gabriel Almeida, Jyväskylä (FI); and Elina Laanto, Jyväskylä (FI)
Assigned to Jyväskylän yliopisto, Jyväskylän yliopisto (FI)
Appl. No. 16/966,472
Filed by Jyväskylän yliopisto, Jyväskylän yliopisto (FI)
PCT Filed Jan. 31, 2019, PCT No. PCT/FI2019/050073 § 371(c)(1), (2) Date Jul. 31, 2020, PCT Pub. No. WO2019/150003, PCT Pub. Date Aug. 8, 2019.
Claims priority of application No. 20185086 (FI), filed on Jan. 31, 2018.
Prior Publication US 2021/0047625 A1, Feb. 18, 2021
Int. Cl. C12N 7/00 (2006.01)

CPC C12N 7/00 (2013.01) [C12N 2795/00021 (2013.01); C12N 2795/00051 (2013.01)]

11 Claims

1. A method for producing bacteriophages comprising the steps of:

a) adding bacterial cells to a sterile culture medium supplemented with mucin,

b) incubating said culture medium, thereby obtaining a bacterial culture modified by mucin exposure,

c) optionally removing a biofilm from said bacterial culture,

d) adding a bacteriophage capable of infecting the bacterial cells to the bacterial culture obtained from step b) or c), and

e) incubating the bacterial culture obtained in step d) until the culture is ready for phage collection.