	US 12,110,509 B2
	Stem cell-derived Sertoli-like cell, preparation method therefor, and use thereof
Dong Ryul Lee, Seoul (KR); and Dong Won Seol, Yongin-si (KR)
Assigned to CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, Pocheon-si (KR)
Filed by CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, Pocheon-si (KR)
Filed on Mar. 6, 2020, as Appl. No. 16/811,086.
Application 16/811,086 is a continuation in part of application No. PCT/KR2018/010525, filed on Sep. 7, 2018.
Claims priority of application No. 10-2017-0114680 (KR), filed on Sep. 7, 2017.
Prior Publication US 2020/0239853 A1, Jul. 30, 2020
Int. Cl. C12N 5/071 (2010.01); C12N 5/074 (2010.01)

CPC C12N 5/0683 (2013.01) [C12N 5/0696 (2013.01); C12N 2501/115 (2013.01); C12N 2501/84 (2013.01); C12N 2506/1346 (2013.01)]

6 Claims

1. A method of preparing Sertoli-like cells (SLC) from stem cells, the method comprising:

inducing differentiation into intermediate mesoderm (IM) by culturing stem cells in a medium,

wherein the inducing differentiation into the IM comprises culturing stem cells for one day to three days in the medium comprising a glycogen synthase kinase 3 (GSK-3) inhibitor, and additionally culturing for two days to five days after adding a base fibroblast growth factor (bFGF) and retinoic acid (RA) to the medium; and

inducing differentiation into SLC by culturing the induced IM in a medium comprising bFGF, fibroblast growth factor 9 (FGF9), prostaglandin D2 (PDG2), follicle-stimulating hormone (FSH), and a glial cell-induced neurotrophic factor (GDNF),

wherein the stem cells are embryonic stem cells (ESC).