US 12,110,506 B2
Method for culturing tumor infiltrating lymphocytes
Yarong Liu, Shanghai (CN); and Peipei Zhao, Shanghai (CN)
Assigned to SUZHOU GRIT BIOTECHNOLOGY CO., LTD., Shanghai (CN); SHANGHAI GRIT BIOTECHNOLOGY CO., LTD., Shanghai (CN); ZHUHAI TUOYU BIOTECHNOLOGY CO., LTD., Shanghai (CN); and SHENZHEN GRIT BIOTECHNOLOGY CO., LTD., Shanghai (CN)
Filed by SUZHOU GRIT BIOTECHNOLOGY CO., LTD., Shanghai (CN); SHANGHAI GRIT BIOTECHNOLOGY CO., LTD., Shanghai (CN); ZHUHAI TUOYU BIOTECHNOLOGY CO., LTD., Shanghai (CN); and SHENZHEN GRIT BIOTECHNOLOGY CO., LTD., Shanghai (CN)
Filed on May 18, 2023, as Appl. No. 18/319,901.
Application 18/319,901 is a continuation of application No. PCT/CN2021/131377, filed on Nov. 18, 2021.
Claims priority of application No. 202011303703.3 (CN), filed on Nov. 19, 2020.
Prior Publication US 2023/0303977 A1, Sep. 28, 2023
Int. Cl. C12N 5/0783 (2010.01); A61K 35/17 (2015.01); A61P 35/00 (2006.01)
CPC C12N 5/0636 (2013.01) [A61K 35/17 (2013.01); A61P 35/00 (2018.01); C12N 2501/2302 (2013.01); C12N 2501/515 (2013.01); C12N 2502/11 (2013.01)] 24 Claims
 
1. A method for culturing tumor infiltrating lymphocytes (TILs), wherein the method comprises subjecting TILs that are derived from tumor tissues and not expanded in vitro to at least one stage of in vitro expansion, and wherein a single stage of the in vitro expansion comprises co-culturing non-in vitro-expanded or in vitro-expanded TILs with feeder cells after contacting the non-in vitro-expanded or in vitro-expanded TILs with one or more T cell co-stimulatory molecules and one or more T cell growth factors for a period of time, wherein the period of time is 12 hours or more.