US 12,110,505 B2
Method for preparing a leukocyte preparation and leukocyte preparation
Jens Altrichter, Dierhagen (DE); Steffen Mitzner, Rostock (DE); Antje Schwarz, Schwerin (DE); Kathleen Selleng, Greifswald (DE); Fanny Doss, Rostock (DE); and Stephanie Koch, Barsinghausen (DE)
Assigned to ARTCLINE GMBH, Rostock (DE)
Filed by ARTCLINE GMBH, Rostock (DE); and FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V., Munich (DE)
Filed on Jul. 9, 2019, as Appl. No. 16/506,462.
Application 16/506,462 is a continuation of application No. 14/563,474, filed on Dec. 8, 2014, granted, now 10,377,987.
Application 14/563,474 is a continuation of application No. PCT/EP2013/001664, filed on Jun. 6, 2013.
Claims priority of application No. 10 2012 209 673.6 (DE), filed on Jun. 8, 2012.
Prior Publication US 2020/0002673 A1, Jan. 2, 2020
Int. Cl. C12N 5/0783 (2010.01); A61K 35/16 (2015.01)
CPC C12N 5/0636 (2013.01) [A61K 35/16 (2013.01)] 17 Claims
 
1. A leukocyte preparation comprising:
leukocytes;
erythrocytes; and
thrombocytes;
wherein the leukocyte preparation is produced according to a method comprising:
obtaining a leukocyte fraction from whole blood;
sedimenting the leukocyte fraction until a dividing line is formed between sedimented erythrocytes and a leukocyte supernatant, removing the leukocyte supernatant from the sediment, wherein the leukocyte supernatant is collected or remains in a leukocyte container;
sedimenting the leukocytes in the leukocyte container, wherein a low-leukocyte supernatant is formed, and removing the low-leukocyte supernatant from the leukocyte container;
washing the sediment in the leukocyte container with a saline solution, wherein the sedimented cells are resuspended in the saline solution, the solution is sedimented and a resulting supernatant is removed;
resuspending the sediment in the leukocyte container in a storage solution; and
storing the solution from the resuspending in a gas-permeable bag at a temperature between 20° C. and 25° C. for at least 48 hours;
wherein the method modifies the leukocyte preparation to create a leukocyte preparation that is storable at a temperature between 2° C. and 38° C. for a period of 48 hours to 168 hours without substantial loss of function and respiratory burst and phagocytosis are more than 90 percent of a fresh version of the leukocyte preparation.