US 12,110,503 B2
Method for differentiation of ocular cells and use thereof
Lucas Chase, Madison, WI (US); Kyle Wallace, Madison, WI (US); Benjamin Meline, Madison, WI (US); Andrew Dias, Madison, WI (US); Brandon Shelley, Madison, WI (US); Marisa Fenn, Madison, WI (US); Debjani Phillips, Madison, WI (US); Matthew Sternfeld, Madison, WI (US); and Nathan Meyer, Madison, WI (US)
Assigned to FUJIFILM Cellular Dynamics, Inc., Madison, WI (US)
Appl. No. 17/049,018
Filed by FUJIFILM Cellular Dynamics, Inc., Madison, WI (US)
PCT Filed Apr. 22, 2019, PCT No. PCT/US2019/028557
§ 371(c)(1), (2) Date Oct. 19, 2020,
PCT Pub. No. WO2019/204817, PCT Pub. Date Oct. 24, 2019.
Claims priority of provisional application 62/660,899, filed on Apr. 20, 2018.
Prior Publication US 2021/0238546 A1, Aug. 5, 2021
Int. Cl. C12N 5/079 (2010.01); A61K 35/30 (2015.01); C12N 5/0793 (2010.01); G01N 33/68 (2006.01)
CPC C12N 5/0621 (2013.01) [A61K 35/30 (2013.01); C12N 5/062 (2013.01); G01N 33/68 (2013.01); C12N 2500/38 (2013.01); C12N 2501/105 (2013.01); C12N 2501/115 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/415 (2013.01); C12N 2501/727 (2013.01); C12N 2501/734 (2013.01); C12N 2506/45 (2013.01)] 29 Claims
 
1. A method for producing a population of neural retinal progenitors (NRPs) comprising:
(a) obtaining a starting population of human induced pluripotent stem cells (iPSCs);
(b) culturing the cells in a first retinal differentiation medium (RD1) comprising a BMP inhibitor to further differentiate the cells to anterior neuroectoderm cells;
(c) inducing retinal differentiation of the anterior neuroectoderm cells by culturing the cells in a second retinal differentiation medium (RD2) essentially free of BMP inhibitors to form retinal progenitor cells (RPCs); and
(d) culturing the RPCs in a retinal maturation (RM) medium to produce NRPs.