US 12,110,497 B2
Pseudotyped insect baculovirus gene transfer system and pseudotyped baculovirus for shrimps, construction method and use thereof
Huarong Guo, Shandong (CN); and Mengxi Wu, Shandong (CN)
Assigned to OCEAN UNIVERSITY OF CHINA, Shandong (CN)
Filed by Ocean University of China, Shandong (CN)
Filed on Aug. 19, 2020, as Appl. No. 16/997,231.
Claims priority of application No. 202010196617.0 (CN), filed on Mar. 19, 2020.
Prior Publication US 2021/0292787 A1, Sep. 23, 2021
Int. Cl. C12N 15/86 (2006.01); C12N 7/00 (2006.01)
CPC C12N 15/86 (2013.01) [C12N 7/00 (2013.01); C12N 2710/14043 (2013.01); C12N 2800/105 (2013.01)] 4 Claims
 
1. A construction method of a pseudotyped insect baculovirus for shrimps based on a gene transfer system, comprising the following steps:
1) constructing, with a Bac-to-Bac insect baculovirus expression system, an insect baculovirus recombinant plasmid comprising a foreign gene; and
2) co-transfecting the insect baculovirus recombinant plasmid obtained in step 1) and an expression plasmid carrying shrimp virus envelope protein gene into an insect packaging cell to obtain a pseudotyped insect baculovirus for shrimps;
wherein the gene transfer system for shrimps comprises a Bac-to-Bac insect baculovirus expression system, a shrimp virus envelope protein gene expression plasmid and an insect packaging cell;
wherein the envelope protein gene in the shrimp virus envelope protein gene expression plasmid has a nucleotide sequence as shown in SEQ ID NO. 1.