US 12,110,493 B2
Engineered purine nucleoside phosphorylase variant enzymes
Scott J. Novick, Palo Alto, CA (US); Nikki Dellas, San Carlos, CA (US); Vesna Mitchell, Santa Clara, CA (US); Da Duan, Newark, CA (US); Jovana Nazor, Milpitas, CA (US); Oscar Alvizo, Fremont, CA (US); Auric Anthony Sowell-Kantz, Mill Valley, CA (US); Jeffrey C. Moore, Westfield, NJ (US); Mark A. Huffman, Rahway, NJ (US); Agustina Rodriguez-Granillo, Rahway, NJ (US); Deeptak Verma, Rahway, NJ (US); Nicholas M. Marshall, Rahway, NJ (US); Jay H. Russell, Rahway, NJ (US); and Keith A. Canada, Freehold, NJ (US)
Assigned to Codexis, Inc., Redwood City, CA (US)
Filed by Codexis, Inc., Redwood City, CA (US)
Filed on Sep. 30, 2021, as Appl. No. 17/490,215.
Application 17/490,215 is a division of application No. 16/460,025, filed on Jul. 2, 2019, granted, now 11,162,105.
Claims priority of provisional application 62/695,507, filed on Jul. 9, 2018.
Claims priority of provisional application 62/822,263, filed on Mar. 22, 2019.
Prior Publication US 2022/0010316 A1, Jan. 13, 2022
Int. Cl. C12N 15/63 (2006.01)
CPC C12N 15/63 (2013.01) [C12Y 204/02001 (2013.01)] 10 Claims
 
1. A polynucleotide sequence encoding an engineered purine nucleoside phosphorylase comprising a polypeptide sequence having at least 85% sequence identity to SEQ ID NO:2, wherein the polypeptide sequence of said engineered purine nucleoside phosphorylase comprises a substitution at position 65 and wherein the polypeptide sequence is numbered with reference to SEQ ID NO: 2, and wherein said engineered purine nucleoside phosphorylase comprises improved activity on compound 3

OG Complex Work Unit Chemistry
as compared to wild-type E. coli purine nucleoside phosphorylase.