US 12,110,483 B2
Perfusion bioreactor with filtration systems
Jiuyi Lu, Topsfield, MA (US); Jason Walther, Holliston, MA (US); Jonathan Wang, Brighton, MA (US); and Kevin Victor Chen, Marlborough, MA (US)
Assigned to Genzyme Corporation, Cambridge, MA (US)
Filed by Genzyme Corporation, Cambridge, MA (US)
Filed on Apr. 28, 2023, as Appl. No. 18/308,870.
Application 18/308,870 is a continuation of application No. 16/403,161, filed on May 3, 2019, abandoned.
Claims priority of provisional application 62/667,319, filed on May 4, 2018.
Prior Publication US 2023/0340396 A1, Oct. 26, 2023
Int. Cl. B01D 15/08 (2006.01); B01D 15/18 (2006.01); B01D 61/18 (2006.01); B01D 61/22 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12M 1/36 (2006.01)
CPC C12M 41/48 (2013.01) [B01D 15/1807 (2013.01); B01D 61/18 (2013.01); B01D 61/22 (2013.01); C12M 29/04 (2013.01); C12M 29/10 (2013.01); C12M 41/40 (2013.01); B01D 2311/2626 (2013.01); B01D 2311/2688 (2013.01); B01D 2313/243 (2013.01); B01D 2315/10 (2013.01); B01D 2317/04 (2013.01); B01D 2317/06 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A perfusion cell culture apparatus comprising:
(a) a bioreactor vessel configured to receive liquid media comprising a cell culture;
(b) a first filtration assembly in fluid communication with the bioreactor vessel, wherein the first filtration assembly comprises:
(1) a first filtration system;
(ii) a first harvest pump connected in series with the first filtration system, wherein the first harvest pump is configured to pump the liquid media from the bioreactor vessel through the first filtration system; and
(iii) a sensor, wherein the sensor is configured to interact with the liquid media inside the first filtration assembly; and
(c) a second filtration assembly configured to operate in parallel with the first filtration assembly, wherein the second filtration assembly is in fluid communication with the bioreactor vessel, and wherein the second filtration assembly comprises:
(i) a second filtration system; and
(ii) a second harvest pump connected in series with the second filtration system, wherein the second harvest pump is configured to pump the liquid media from the bioreactor vessel through the second filtration system; and
(d) a controller, wherein the controller executes operations comprising:
(i) receiving, from the sensor, information indicative of changes in cell density in the first filtration system;
(ii) determining, based on at least the received information from the sensor, changes in cell density in the first filtration system;
(iii) responsive to a determination that of changes in cell density in the first filtration system, causing the first harvest pump to stop pumping liquid media through the first filtration system; and
(iv) responsive to the determination of changes in cell density in the first filtration system, causing the second harvest pump to increase a flow rate of liquid media through the second filtration system.