US 12,110,471 B2
Enzymatic removal of chlorophyll substrates from triacylglycerol-based oils
Christopher Loren Gene Dayton, Jupiter, FL (US); Arjen Sein, Echt (NL); Evert Tjeerd Van Rij, Echt (NL); and Jan Metske Van Der Laan, Echt (NL)
Assigned to Bunge Global Innovation, LLC, Chesterfield, MO (US)
Filed by Bunge Global Innovation, LLC, Chesterfield, MO (US)
Filed on Aug. 15, 2022, as Appl. No. 17/819,652.
Application 17/819,652 is a continuation of application No. 16/650,599, granted, now 11,447,714, previously published as PCT/EP2018/076130, filed on Sep. 26, 2018.
Claims priority of provisional application 62/736,126, filed on Sep. 25, 2018.
Claims priority of provisional application 62/736,129, filed on Sep. 25, 2018.
Claims priority of application No. 17193211 (EP), filed on Sep. 26, 2017.
Prior Publication US 2022/0380700 A1, Dec. 1, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/16 (2006.01); C11B 3/00 (2006.01); C12N 9/18 (2006.01)
CPC C11B 3/003 (2013.01) [C12N 9/18 (2013.01); C12Y 301/01014 (2013.01); C12Y 301/04003 (2013.01)] 29 Claims
 
1. A process for treating an oil comprising a chlorophyll substrate, the process comprising contacting the oil with a polypeptide having decolorase activity or a composition comprising the polypeptide, wherein the polypeptide is dosed into the oil in an amount of 1 to 50 U/gram oil, wherein the treatment reduces the total concentration of chlorophyll substrates in the oil by at least 5% by weight, compared to the total concentration of chlorophyll substrates in the oil prior to treatment.