US 12,110,331 B2
Canine monoclonal antibodies against canine cytotoxic T lymphocyte associated protein 4 (CTLA-4)
Nicola J. Mason, Philadelphia, PA (US); Donald L. Siegel, Lansdale, PA (US); and Nicholas Chester, Philadelphia, PA (US)
Assigned to The Trustees of the University of Pennsylvania, Philadelphia, PA (US); and Vetigenics, Inc., Philadelphia, PA (US)
Filed by THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA, Philadelphia, PA (US); and VETIGENICS, INC., Philadelphia, PA (US)
Filed on Oct. 20, 2023, as Appl. No. 18/491,182.
Application 18/491,182 is a continuation of application No. PCT/US2022/025614, filed on Apr. 20, 2022.
Claims priority of provisional application 63/177,692, filed on Apr. 21, 2021.
Prior Publication US 2024/0109967 A1, Apr. 4, 2024
Int. Cl. C07K 16/28 (2006.01)
CPC C07K 16/2818 (2013.01) [C07K 2317/20 (2013.01); C07K 2317/622 (2013.01); C07K 2317/76 (2013.01); C07K 2317/92 (2013.01)] 20 Claims
 
1. An antibody or antigen-binding fragment thereof comprising an antigen-binding domain that specifically binds to an epitope of canine cytotoxic T lymphocyte-associated protein 4 (CTLA-4), wherein the antigen-binding domain comprises:
(i) a heavy chain variable region comprising an amino acid sequence having at least 80% identity to the amino acid sequence of the heavy chain variable region set forth in SEQ ID NO: 6; and
(ii) a light chain variable region comprising an amino acid sequence having at least 80% identity to the amino acid sequence set forth in SEQ ID NO: 8;
wherein the heavy chain variable region comprises three heavy chain complementarity determining regions (HCDRs), wherein HCDR1 comprises the amino acid sequence set forth in SEQ ID NO: 1, HCDR2 comprises the amino acid sequence set forth in SEQ ID NO: 2, and HCDR3 comprises the amino acid sequence set forth in of SEQ ID NO: 3; and
wherein the light chain variable region comprises three light chain complementarity determining regions (LCDRs), wherein LCDR1 comprises the amino acid sequence set forth in SEQ ID NO: 4, LCDR2 comprises the amino acid sequence of VDG, and LCDR3 comprises the amino acid sequence set forth in SEQ ID NO: 5.