	US 12,110,313 B2
	Engineered bacterial ribosome compositions and methods
Sidney Hecht, Phoenix, AZ (US); Larisa Dedkova, Scottsdale, AZ (US); Shengxi Chen, Chandler, AZ (US); and Xiaoguang Bai, Scottsdale, AZ (US)
Assigned to ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US)
Filed by Sidney Hecht, Phoenix, AZ (US); Larisa Dedkova, Scottsdale, AZ (US); Shengxi Chen, Chandler, AZ (US); and Xiaoguang Bai, Scottsdale, AZ (US)
Filed on Feb. 28, 2020, as Appl. No. 16/804,691.
Claims priority of provisional application 62/811,638, filed on Feb. 28, 2019.
Prior Publication US 2020/0299333 A1, Sep. 24, 2020
Int. Cl. C07K 14/00 (2006.01); C07H 19/16 (2006.01); C07K 14/565 (2006.01)

CPC C07K 14/001 (2013.01) [C07H 19/16 (2013.01); C07K 14/565 (2013.01)]

11 Claims

1. An Escherichia coli (E. coli) bacterial ribosome comprising a modified 23S ribosomal RNA sequence comprising modifications as compared to a wild-type counterpart, wherein the modifications consist of a first modified region and a second modified region;

wherein the 23S ribosomal RNA is expressed from an engineered rrnB operon;

wherein the first modified region consists of the sequence UGCGUGG from position 2057 to 2063;

wherein the second modified region consists of a sequence selected from GGGAAG and UCGAGA from position 2496 to 2501;

wherein a DNA sequence encoding a first unmodified region of the wild-type counterpart consists of the sequence GAAAGAC from position 2057 to 2063; and

wherein a second unmodified region of the wild-type counterpart consists of the sequence CACCUC from position 2496 to 2501.