US 12,109,566 B2
Cell concentration devices and methods including a syringe and a syringe holder
Andy H. Levine, Newton Highlands, MA (US); John C. Meade, Mendon, MA (US); Neil Tischler, Acton, MA (US); Jeffrey R. Chabot, Medford, MA (US); Andrew McGillicuddy, Humarock, MA (US); and Neil F. Duffy, Jr., Brighton, MA (US)
Assigned to Cervos Medical LLC, Avon, MA (US)
Filed by Cervos Medical LLC, Marshfield, MA (US)
Filed on May 18, 2023, as Appl. No. 18/320,038.
Application 16/835,053 is a division of application No. 14/764,115, granted, now 10,603,665, issued on Mar. 31, 2020, previously published as PCT/US2014/013636, filed on Jan. 29, 2014.
Application 18/320,038 is a continuation of application No. 17/715,222, filed on Apr. 7, 2022, granted, now 11,660,603.
Application 17/715,222 is a continuation of application No. 16/835,053, filed on Mar. 30, 2020.
Claims priority of provisional application 61/897,587, filed on Oct. 30, 2013.
Claims priority of provisional application 61/757,993, filed on Jan. 29, 2013.
Prior Publication US 2024/0123449 A1, Apr. 18, 2024
Int. Cl. B01L 3/00 (2006.01); A61M 1/02 (2006.01); A61M 1/36 (2006.01); B01D 21/26 (2006.01); B01L 3/02 (2006.01)
CPC B01L 3/5082 (2013.01) [A61M 1/029 (2013.01); A61M 1/3693 (2013.01); B01D 21/262 (2013.01); B01L 3/0282 (2013.01); B01L 3/50215 (2013.01); B01L 2200/026 (2013.01); B01L 2200/0684 (2013.01); B01L 2200/0689 (2013.01); B01L 2300/046 (2013.01); B01L 2300/0858 (2013.01); B01L 2400/0409 (2013.01); B01L 2400/0478 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A system for separating components of different densities from a fluid containing cells using a centrifuge, the system comprising:
a syringe having a top, a sidewall extending from the top forming a syringe tube, and a plunger slidably disposed inside the syringe tube and in sealing engagement with the sidewall, the sidewall being substantially straight from the top to a distal end of the syringe tube, the distal end of the syringe tube including an opening, the syringe defining a cavity for receiving the fluid containing cells;
a Luer port disposed in the top of the syringe to transfer the fluid containing cells into the syringe and to extract one or more separated layers containing cells from the syringe; and
a syringe holder at least partially closing the opening of the distal end of the syringe tube, the syringe holder not connected to the plunger inside the syringe tube,
the syringe and syringe holder configured for placement in a centrifuge and for exposure of the syringe and syringe holder to elevated g force in the centrifuge, the elevated g force urging the plunger toward the syringe holder, wherein the syringe holder is in physical contact with the syringe tube on the distal end and includes a surface arranged to contact the plunger at a bottom perimeter of the plunger during centrifugation, to provide support and maintain the sealing engagement of the plunger with the sidewall of the syringe tube, to prevent fluid from leaking outside the syringe.