US 12,109,440 B2
Compositions, methods and systems for gas vesicle based cavitation
Avinoam D. Bar-Zion, Pasadena, CA (US); Atousa Nourmahnad, Pasadena, CA (US); David Maresca, Pasadena, CA (US); and Mikhail Shapiro, Pasadena, CA (US)
Assigned to CALIFORNIA INSTITUTE OF TECHNOLOGY, Pasadena, CA (US)
Filed by California Institute of Technology, Pasadena, CA (US)
Filed on Jul. 29, 2022, as Appl. No. 17/816,373.
Application 17/816,373 is a continuation of application No. 16/833,637, filed on Mar. 29, 2020, granted, now 11,446,523.
Claims priority of provisional application 62/825,612, filed on Mar. 28, 2019.
Claims priority of provisional application 62/895,553, filed on Sep. 4, 2019.
Prior Publication US 2023/0139561 A1, May 4, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A61N 7/00 (2006.01)
CPC A61N 7/00 (2013.01) [A61N 2007/0004 (2013.01); A61N 2007/0039 (2013.01); A61N 2007/0052 (2013.01); A61N 2007/0082 (2013.01)] 35 Claims
OG exemplary drawing
 
1. An ultrasound pressure-based method comprising:
delivering a gas vesicle (GV) to a target site, the GV having a GV type collapse threshold pressure; setting an ultrasound generation system to produce ultrasound pulses having a positive pressure set above the GV type collapse threshold pressure, a negative pressure and at least one of: a duty cycle above 0.2% or a mechanical index above 2.8; and applying the ultrasound pulses to the target site, thus: collapsing the GV by the positive pressure of the ultrasound pulses, thereby forming gas bubbles; and
driving a cavitation of the gas bubbles by the negative pressure wherein the gas vesicle comprises a variant GvpC engineered to comprise binding tags presented on the GV and configured to bind to a target molecule on a cavitation target.