US 12,109,327 B2
Scaffolds from self-assembling tetrapeptides support 3D spreading, osteogenic differentiation and angiogenesis of mesenchymal stem cells
Charlotte A. E. Hauser, Thuwal (SA); Salwa Alshehri, Thuwal (SA); and Hepi H. Susapto, Thuwal (SA)
Assigned to KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY, Thuwal (SA)
Filed by KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY, Thuwal (SA)
Filed on Aug. 13, 2021, as Appl. No. 17/401,542.
Claims priority of provisional application 63/067,962, filed on Aug. 20, 2020.
Claims priority of provisional application 63/067,913, filed on Aug. 20, 2020.
Prior Publication US 2022/0054706 A1, Feb. 24, 2022
Int. Cl. A61L 27/36 (2006.01); A61L 27/22 (2006.01); B33Y 10/00 (2015.01); B33Y 70/00 (2020.01); B33Y 80/00 (2015.01); C12N 5/077 (2010.01)
CPC A61L 27/365 (2013.01) [A61L 27/22 (2013.01); B33Y 10/00 (2014.12); B33Y 70/00 (2014.12); B33Y 80/00 (2014.12); C12N 5/0654 (2013.01); A61L 2430/02 (2013.01); C12N 2506/1353 (2013.01); C12N 2513/00 (2013.01); C12N 2533/50 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A 3-dimensional osteo-tissue graft comprising:
live mesenchymal stem cells (MSCs); and
ultrashort self-assembling peptide scaffolds,
wherein the stiffness of the peptide scaffolds is 16-25 kPa,
wherein at least one osteogenic marker selected from the group consisting of bone morphogenetic protein (BMP-2) bone sialoprotein 2 (IBSP), osteopontin (OPN), osterix (OSX), RUNX2, osteocalcin and alkaline phosphatase (ALP), is expressed.