	US 12,109,300 B2
	Method for preparing enzyme-treated Zizania latifolia Turcz. extract having increased tricin content, and composition for whitening, wrinkle reduction, anti-inflammation, anti-allergy and moisturization, prepared thereby
Tae Young Kim, Ansan-si (KR); Joo Myung Moon, Ansan-si (KR); and Sung Kwan Jo, Seoul (KR)
Assigned to BTC CORPORATION, Ansan-si (KR)
Appl. No. 17/049,025
Filed by BTC CORPORATION, Ansan-si (KR)
PCT Filed Sep. 3, 2019, PCT No. PCT/KR2019/011352 § 371(c)(1), (2) Date Oct. 19, 2020, PCT Pub. No. WO2020/122360, PCT Pub. Date Jun. 18, 2020.
Claims priority of application No. 10-2018-0160061 (KR), filed on Dec. 12, 2018.
Prior Publication US 2021/0251883 A1, Aug. 19, 2021
Int. Cl. A61K 36/00 (2006.01); A23L 29/00 (2016.01); A23L 33/00 (2016.01); A23L 33/105 (2016.01); A61K 8/49 (2006.01); A61K 8/9794 (2017.01); A61K 31/353 (2006.01); A61K 36/899 (2006.01); A61P 29/00 (2006.01); A61P 37/08 (2006.01); A61Q 19/02 (2006.01); A61Q 19/08 (2006.01)

CPC A61K 8/9794 (2017.08) [A23L 29/06 (2016.08); A23L 33/105 (2016.08); A23L 33/40 (2016.08); A61K 8/498 (2013.01); A61K 31/353 (2013.01); A61K 36/899 (2013.01); A61P 29/00 (2018.01); A61P 37/08 (2018.01); A61Q 19/02 (2013.01); A61Q 19/08 (2013.01); A23V 2002/00 (2013.01); A61K 2236/19 (2013.01); A61K 2236/333 (2013.01); A61K 2800/805 (2013.01)]

4 Claims

1. A method of enhancing skin activities, comprising administering an effective amount of a cosmetic composition comprising an enzyme-treated Zizania latifolia Turcz. extract prepared by the method comprising the steps of:

(1) adding water to Zizania latifolia Turcz. to produce a solution and heating for a sufficient duration of time;

(2) cooling heated solution and treating with one or more enzyme(s) selected from the group consisting of: α-amylase, β-galactosidase, xylanase, lactase, lipase, tannase, cellulase, hemicellulase, pectinase and β-glucosidase, to produce an enzyme-treated solution;

(3) filtering the enzyme-treated solution to obtain a primary extract and a remaining residue;

(4) extracting the remaining residue after filtering in step (3) with any solvent selected from the group consisting of: water, lower alcohols of C1 to C4 and mixtures thereof to obtain a secondary extract; and

(5) mixing the primary and secondary extracts and concentrating or drying to produce the enzyme-treated Zizania latifolia Turcz. extract;

wherein the enzyme-treated Zizania latifolia Turcz. extract contains 0.01% to 90% by weight of tricin.