	US 12,109,276 B2
	Near-infrared dyes and conjugates for targeting tumors
Lyle D. Small, Colorado Springs, CO (US); Ruizheng Wang, Colorado Springs, CO (US); and Shane M. Crippen, Colorado Springs, CO (US)
Assigned to Lahjavida, LLC, Colorado Springs, CO (US)
Filed by Lahjavida, LLC, Colorado Springs, CO (US)
Filed on Mar. 11, 2020, as Appl. No. 16/816,118.
Claims priority of provisional application 62/819,146, filed on Mar. 15, 2019.
Prior Publication US 2020/0289676 A1, Sep. 17, 2020
Int. Cl. A61B 5/00 (2006.01); A61B 8/00 (2006.01); A61B 10/00 (2006.01); A61K 9/51 (2006.01); A61K 31/704 (2006.01); A61K 49/00 (2006.01); C09B 23/01 (2006.01)

CPC A61K 49/0032 (2013.01) [A61K 9/51 (2013.01); A61K 31/704 (2013.01); A61K 49/0056 (2013.01); C09B 23/0066 (2013.01)]

12 Claims

1. A method of treating or killing tumor cells, comprising:

administering a tumor-targeting conjugate to a subject, said conjugate comprising a heptamethine cyanine dye covalently bound to a magnetic nanoparticle via a linker;

wherein the tumor-targeting component of said conjugate consists of said heptamethine cyanine dye; and

inducing hyperthermia in said tumor cells via electromagnetic radiation having a frequency comprising kilohertz or megahertz;

wherein said linker comprises one of the following compositions:

wherein n is 3, 5, 8, 12, 22, 45, or 113.