US 12,109,275 B2
Nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal for increasing the expression of an encoded protein
Andreas Thess, Kusterdingen (DE); Thomas Schlake, Gundelfingen (DE); and Jochen Probst, Wolfschlugen (DE)
Assigned to CureVac SE, Tübingen (DE)
Filed by CureVac SE, Tübingen (DE)
Filed on Apr. 15, 2020, as Appl. No. 16/848,991.
Application 16/848,991 is a continuation of application No. 15/810,080, filed on Nov. 12, 2017, granted, now 10,653,799.
Application 15/810,080 is a continuation of application No. 14/945,349, filed on Nov. 18, 2015, granted, now 9,839,697, issued on Dec. 12, 2017.
Application 14/945,349 is a continuation of application No. 13/321,474, granted, now 9,234,013, issued on Jan. 12, 2016, previously published as PCT/EP2011/004077, filed on Aug. 12, 2011.
Claims priority of application No. PCT/EP2010/004998 (WO), filed on Aug. 13, 2010.
Prior Publication US 2020/0276336 A1, Sep. 3, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 48/00 (2006.01); A61K 39/00 (2006.01); C07K 14/435 (2006.01); C12N 15/67 (2006.01)
CPC A61K 48/0066 (2013.01) [A61K 39/0011 (2013.01); C07K 14/435 (2013.01); C12N 15/67 (2013.01); A61K 2039/53 (2013.01); A61K 2039/575 (2013.01)] 19 Claims
 
1. A purified RNA molecule comprising:
a) a coding region encoding a polypeptide;
b) at least one histone stem-loop comprising the RNA sequence of SEQ ID NO: 27; and
c) a poly(A) sequence of about 25 to about 400 adenosine nucleotides.