US 12,109,274 B2
Polynucleotides containing a stabilizing tail region
Gabor Butora, Cambridge, MA (US); Andrew W. Fraley, Arlington, MA (US); Edward John Miracco, Cambridge, MA (US); Jennifer Nelson, Brookline, MA (US); Amy Rhoden Smith, Watertown, MA (US); Matthew Stanton, Marlton, NJ (US); and Brian R. Fritz, Cambridge, MA (US)
Assigned to ModernaTX, Inc., Cambridge, MA (US)
Appl. No. 15/760,686
Filed by ModernaTX, Inc., Cambridge, MA (US)
PCT Filed Sep. 19, 2016, PCT No. PCT/US2016/052451
§ 371(c)(1), (2) Date Mar. 16, 2018,
PCT Pub. No. WO2017/049275, PCT Pub. Date Mar. 23, 2017.
Claims priority of provisional application 62/279,466, filed on Jan. 15, 2016.
Claims priority of provisional application 62/220,050, filed on Sep. 17, 2015.
Prior Publication US 2018/0256750 A1, Sep. 13, 2018
Int. Cl. A61K 48/00 (2006.01); C07F 9/6558 (2006.01); C07F 9/6561 (2006.01); C07H 21/02 (2006.01); C12N 15/67 (2006.01)
CPC A61K 48/0066 (2013.01) [C07F 9/65586 (2013.01); C07F 9/65616 (2013.01); C07H 21/02 (2013.01); C12N 15/67 (2013.01)] 9 Claims
 
1. A polynucleotide encoding a polypeptide, wherein said polynucleotide comprises:
(a) a 5′-cap structure;
(b) a 5′-UTR;
(c) a coding region encoding the polypeptide;
(d) a 3′-UTR; and
(e) a 3′-stabilizing region comprising 1 to 500 nucleosides, wherein one or more of said nucleosides is an inverted thymidine.