1. A method for producing human hemogenic endothelial (PVE-HE) cells comprising culturing human pluripotent stem cells in vitro on an extracellular matrix, under feeder free conditions, and in a differentiation induction medium (DIM) comprising BMP4, bFGF and VEGF for at least 4 days, wherein the at least 4 days comprise a first period of low oxygen conditions comprising 1% to 10% oxygen and a second period of normoxic conditions, thereby forming a cell population comprising hemogenic endothelial cells, wherein the human pluripotent stem cells are differentiated into the hemogenic endothelial cells without embryoid body formation.
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