	US 12,109,224 B2
	USB1 directed regulation of miRNAs related hematopoietic differentiation and affected in multiple forms of leukemia
Roy R. Parker, Boulder, CO (US); Siddharth Shukla, Boulder, CO (US); Luis F. Z. Batista, Clayton, MO (US); and Hochang Jeong, St. Louis, MO (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATE, Denver, CO (US); and WASHINGTON UNIVERSITY, St. Louis, MO (US)
Filed by The Regents of the University of Colorado, a body corporate, Denver, CO (US); and Washington University, St. Louis, MO (US)
Filed on Sep. 3, 2021, as Appl. No. 17/466,448.
Claims priority of provisional application 63/074,772, filed on Sep. 4, 2020.
Prior Publication US 2022/0088053 A1, Mar. 24, 2022
Int. Cl. A61K 31/713 (2006.01); A61K 31/4375 (2006.01)

CPC A61K 31/713 (2013.01) [A61K 31/4375 (2013.01)]

2 Claims

1. A method of downregulating miRNA expression in a cell comprising the step of downregulating the expression of usb17 in the cell via introduction of a CRISPR-mediated gene therapy that knocks out usb17 in the cell, and wherein the downregulated miRNA include at least one of the following: miR-142-5p, miR-199a-3p, and miR-223-3p.