US 12,109,223 B2
Polymer nanoparticle and DNA nanostructure compositions and methods for non-viral delivery
Anthony D. Duong, Columbus, OH (US); Cherry Gupta, Columbus, OH (US); Kenneth R. Sims, Jr., Columbus, OH (US); Danielle Huk, Columbus, OH (US); Jacob Lilly, Columbus, OH (US); Stephanie M. Kute, Columbus, OH (US); and Mike Koeris, Columbus, OH (US)
Assigned to BATTELLE MEMORIAL INSTITUTE, Columbus, OH (US)
Filed by BATTELLE MEMORIAL INSTITUTE, Columbus, OH (US)
Filed on Feb. 16, 2024, as Appl. No. 18/444,146.
Application 18/444,146 is a continuation of application No. 17/542,055, filed on Dec. 3, 2021.
Claims priority of provisional application 63/121,202, filed on Dec. 3, 2020.
Prior Publication US 2024/0189338 A1, Jun. 13, 2024
Int. Cl. A61K 47/60 (2017.01); A61K 31/7088 (2006.01); A61K 47/69 (2017.01); C08F 293/00 (2006.01); C12N 15/113 (2010.01); B82Y 5/00 (2011.01); B82Y 30/00 (2011.01); B82Y 35/00 (2011.01); B82Y 40/00 (2011.01)
CPC A61K 31/7088 (2013.01) [A61K 47/60 (2017.08); A61K 47/6935 (2017.08); C08F 293/005 (2013.01); C12N 15/113 (2013.01); B82Y 5/00 (2013.01); B82Y 30/00 (2013.01); B82Y 35/00 (2013.01); B82Y 40/00 (2013.01); C08F 2438/03 (2013.01); C12N 2310/11 (2013.01); C12N 2310/531 (2013.01)] 30 Claims
 
1. A RAFT block copolymer comprising
a. a first terminus comprising a first capping unit derived from a first chain transfer agent in a RAFT copolymerization process;
b. a first block prepared from one or more monomer units covalently attached to the first reactive functional unit, and having a molecular weight (Mn) in the range of about 20 kDa to about 80 kDa and a degree of polymerization in the range of about 20 to about 400;
c. a second block prepared from one or more monomer units covalently attached to the first block, and having a molecular weight (Mn) in the range of about 5 kDa to about 80 kDa and a degree of polymerization in the range of about 10 to about 500; and
d. a second terminus comprising a second capping unit derived from a second chain transfer agent;
wherein the RAFT block copolymer has one or more of an overall molecular weight (Mn) in the range of about 25 kDa to about 160 kDa, and overall degree of polymerization in the range of about 30 to about 900, a size in the range of about of about 10 to about 60 nm, and a maximum corona-to-core ratio (CCR) of about 1 to about 4.