US 12,108,774 B2
Preparation of pulse protein products (“YP810”)
Kevin I. Segall, Winnipeg (CA); Brent E. Green, Warren (CA); and Martin Schweizer, Winnipeg (CA)
Assigned to Burcon Nutrascience (MB) Corp., Winnipeg (CA)
Filed by Burcon Nutrascience (MB) Corp., Winnipeg (CA)
Filed on Oct. 5, 2021, as Appl. No. 17/494,490.
Application 17/494,490 is a continuation of application No. 14/811,052, filed on Jul. 28, 2015, granted, now 11,589,597.
Claims priority of provisional application 62/029,686, filed on Jul. 28, 2014.
Prior Publication US 2022/0022491 A1, Jan. 27, 2022
Int. Cl. A23J 1/14 (2006.01); A23J 3/14 (2006.01); A23J 3/16 (2006.01); A23L 2/66 (2006.01); C07K 1/34 (2006.01); C07K 14/415 (2006.01)
CPC A23J 1/14 (2013.01) [A23J 3/14 (2013.01); A23J 3/16 (2013.01); A23L 2/66 (2013.01); C07K 1/34 (2013.01); C07K 14/415 (2013.01)] 73 Claims
OG exemplary drawing
 
1. A process of producing a pulse protein product having a protein content selected from the group consisting of at least about 60 wt % and at least about 90 wt % (N×6.25) on a dry weight basis, which comprises:
(a) extracting a pulse protein source with water at a pH of about 6 to about 11 to cause solubilization of pulse protein from the protein source and to form an aqueous pulse protein solution,
(b) at least partially separating the aqueous pulse protein solution from residual pulse protein source,
(c) adjusting the pH of the aqueous pulse protein solution to a pH of about 1.5 to about 3.4 to produce an acidified pulse protein solution,
(d) separating the acid insoluble solid material from the acidified pulse protein solution, and
either carrying out steps (e) to (g):
(e) optionally concentrating the acidified pulse protein solution by a selective membrane technique,
(f) optionally diafiltering the optionally concentrated acidified pulse protein solution, and
(g) optionally drying the optionally concentrated and optionally diafiltered pulse protein solution,
or in place of steps (e)-(g):
(h) concentrating and/or diafiltering the acidified pulse protein solution by a selective membrane technique to fractionate the protein component of the acidified pulse protein solution into a first retentate and a first permeate,
(i) optionally drying the first retentate to provide a first pulse protein product,
(j) concentrating and optionally diafiltering the first permeate to provide a second retentate and a second permeate, and
(k) optionally drying the second retentate to provide a second pulse protein product, wherein the process does not comprise a direct addition and use of calcium salt or other salt in extraction of the protein from the protein source material or in any other step.