US 11,781,191 B2
Compositions and method for detecting Mycobacterium riyadhense
Arnab Pain, Thuwal (SA); and Qingtian Guan, Thuwal (SA)
Assigned to KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY, Thuwal (SA)
Filed by King Abdullah University of Science and Technology, Thuwal (SA)
Filed on Aug. 8, 2020, as Appl. No. 16/988,617.
Claims priority of provisional application 62/884,203, filed on Aug. 8, 2019.
Prior Publication US 2021/0102240 A1, Apr. 8, 2021
Int. Cl. C12Q 1/689 (2018.01)
CPC C12Q 1/689 (2013.01) 19 Claims
 
1. A method of detecting Mycobacterium riyadhense (M. riyadhense) in a sample, the method comprising: contacting the sample with one or more primer pairs in a DNA amplification reaction,
wherein M. riyadhense is detected after detecting the presence of an amplified product,
wherein the amplified product has a size ranging from about 166 bp to about 994 bp, and
wherein the primer pairs are selected from the group consisting of SEQ ID NOs: 1 and 2; SEQ ID Nos: 3 and 4; SEQ ID Nos: 5 and 6, SEQ ID Nos: 7 and 8; and variants thereof.