Feng Zhang, Cambridge, MA (US); Bernd Zetsche, Gloucester, MA (US); Jonathan S. Gootenberg, Cambridge, MA (US); Omar O. Abudayyeh, Boston, MA (US); and Ian Slaymaker, Cambridge, MA (US)
Assigned to THE BROAD INSTITUTE, INC., Cambridge, MA (US); MASSACHUSETTS INSTITUTE OF TECHNOLOGY, Cambridge, MA (US); and PRESIDENT AND FELLOWS OF HARVARD COLLEGE, Cambridge, MA (US)
Filed by The Broad Institute, Inc., Cambridge, MA (US); Massachusetts Institute of Technology, Cambridge, MA (US); and President and Fellows of Harvard College, Cambridge, MA (US)
Filed on Apr. 30, 2019, as Appl. No. 16/400,026.
Application 16/400,026 is a continuation of application No. 15/844,608, filed on Dec. 17, 2017, granted, now 10,648,020.
Application 15/844,608 is a continuation in part of application No. PCT/US2016/038181, filed on Jun. 17, 2016.
Application PCT/US2016/038181 is a continuation in part of application No. 14/975,085, filed on Dec. 18, 2015, granted, now 9,790,490, issued on Oct. 17, 2017.
Claims priority of provisional application 62/232,067, filed on Sep. 24, 2015.
Claims priority of provisional application 62/205,733, filed on Aug. 16, 2015.
Claims priority of provisional application 62/201,542, filed on Aug. 5, 2015.
Claims priority of provisional application 62/193,507, filed on Jul. 16, 2015.
Claims priority of provisional application 62/181,739, filed on Jun. 18, 2015.
Claims priority of application No. 16150428 (EP), filed on Jan. 7, 2016.
Prior Publication US 2019/0256900 A1, Aug. 22, 2019
1. A method of targeting a polynucleotide, comprising: contacting a sample that comprises the polynucleotide with a CRISPR-Cas complex comprising (a) a Cas protein that does not comprise an HNH domain or a polynucleotide encoding the Cas protein and (b) an engineered guide without a tracrRNA that is capable of directing sequence-specific binding of the complex to a target sequence of the polynucleotide or a polynucleotide encoding the engineered guide.