	US 11,781,169 B2
	Enzymatic DNA repair
Robert N. Grass, Zurich (CH); Alexander Xavier Christof Kohll, Zurich (CH); and Weida Chen, Zurich (CH)
Assigned to MICROSOFT TECHNOLOGY LICENSING, LLC, Redmond, WA (US)
Filed by MICROSOFT TECHNOLOGY LICENSING, LLC, Redmond, WA (US)
Filed on Oct. 5, 2018, as Appl. No. 16/152,808.
Prior Publication US 2020/0109436 A1, Apr. 9, 2020
Int. Cl. C12Q 1/6806 (2018.01); G06N 3/123 (2023.01)

CPC C12Q 1/6806 (2013.01) [G06N 3/123 (2013.01)]

20 Claims

1. A method of increasing a quantity of PCR-amplifiable synthetic double-stranded DNA comprising natural bases encoding digital information, the method comprising:

determining that the quantity of PCR-amplifiable DNA in the synthetic double-stranded DNA is less than a threshold level, wherein the threshold level is based on a change in the quantity of PCR-amplifiable DNA before and after storage as measured by quantitative PCR;

contacting the synthetic double-stranded DNA with a repair mixture comprising an endonuclease; and

amplifying the synthetic double-stranded DNA by polymerase chain reaction (PCR).