CPC C12N 15/1003 (2013.01) | 6 Claims |
1. A method of separating human sperm DNA from human non-sperm DNA in sexual assault samples comprising:
contacting a sample containing human sperm cells and human non-sperm cells with proteinase-K in an extraction buffer solution comprising t-octylphenoxypolyethoxyethanol, wherein the extraction buffer solution does not contain sodium dodecyl sulfate;
digesting the human non-sperm cells with the proteinase-K;
adding a DNAse I to the same extraction buffer solution in which the non-sperm cells were digested without a centrifugation step or a filtration step with respect to the addition of the DNAse I;
selectively degrading non-sperm DNA from the non-sperm cells with the DNAse I;
incubating the DNAse I and lysing the sperm by adding ethylenediaminetetraacetic (EDTA) and dithiothreitol (DTT) at 56° C. until the DNAse I is inactivated; and
separating sperm DNA from the human sperm for further analysis of the sperm DNA.
|