CPC C12N 5/0636 (2013.01) [C07K 14/7051 (2013.01); C07K 14/70521 (2013.01); C12M 23/42 (2013.01); C12M 29/20 (2013.01); C12M 41/00 (2013.01); C12N 5/0018 (2013.01); C12N 5/163 (2013.01); C12N 15/86 (2013.01); C12N 2510/00 (2013.01); C12N 2740/10041 (2013.01); C12N 2740/15041 (2013.01)] | 17 Claims |
1. A method for automated production of a genetically modified T cell culture, the method comprising:
a) mixing a T cell culture with a magnetic selection reagent;
b) exposing the T cell culture to magnetic separation;
c) activating the T cell culture with an activation reagent selected from an antibody and a dendritic cell to produce an activated T cell culture;
d) transducing within a cell culture chamber the activated T cell culture with a viral vector encoding an ectodomain, a transmembrane domain and an endodomain, to produce a transduced T cell culture;
e) expanding within the cell culture chamber the transduced T cell culture;
f) centrifuging the expanded T cell culture; and
g) harvesting the T cell culture to produce a genetically modified T cell culture,
wherein (a) through (f) are performed within a fully enclosed, automated cell engineering system, and the cell culture chamber has a fixed area; and
wherein expansion of the transduced T cell culture in (c) produces at least 20% more genetically modified T cells than expansion utilizing manual cell culture with a flexible, gas permeable bag and transduction efficiency of the method is at least 20% higher than transduction efficiency utilizing manual cell culture with the flexible, gas permeable bag.
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