US 11,781,104 B2
Ultra-microinjection detection and control device based on lensless imaging and method thereof
Huijun Gao, Harbin (CN); Mingsi Tong, Harbin (CN); Xinghu Yu, Harbin (CN); and Linqi Zhang, Harbin (CN)
Assigned to Harbin Institute of Technology, Harbin (CN)
Filed by Harbin Institute of Technology, Harbin (CN)
Filed on Feb. 20, 2020, as Appl. No. 16/795,696.
Claims priority of application No. 201910446653.5 (CN), filed on May 27, 2019.
Prior Publication US 2020/0377843 A1, Dec. 3, 2020
Int. Cl. A61M 5/00 (2006.01); C12M 1/34 (2006.01); A61M 5/168 (2006.01); A61M 5/32 (2006.01); C12M 1/36 (2006.01); C12N 15/89 (2006.01); G01F 23/292 (2006.01); A61M 5/158 (2006.01)
CPC C12M 41/44 (2013.01) [A61M 5/16831 (2013.01); A61M 5/3286 (2013.01); C12M 41/48 (2013.01); C12N 15/89 (2013.01); G01F 23/292 (2013.01); A61M 2005/1588 (2013.01)] 8 Claims
OG exemplary drawing
 
1. An ultra-microinjection detection and control device based on lensless imaging, wherein the device comprises an injection pump and a needle holder (e); the needle holder (e) is used to hold an injection needle (c); the injection pump is used to provide power needed for injection; the device further comprises a lensless optical liquid level sensor and a microinjection control unit;
the lensless optical liquid level sensor is arranged on an outer wall of the injection needle (c), for measuring a change of a liquid level in the injection needle (c);
the microinjection control unit is arranged on the needle holder (e), for detecting the change of the liquid level and correcting an injection pressure of the injection pump, so as to stabilize the liquid level in the injection needle (c);
the lensless optical liquid level sensor comprises a micro linear array image sensor chip (5), a light filtering film (1), a parallel light source (4) and a holding housing (6);
the parallel light source (4) is arranged on an outer side wall of the injection needle (c); the light filtering film (1) is arranged on an outer side wall of the injection needle (c) and located on a transmitted plane of the parallel light source (4); the micro linear array image sensor chip (5) is arranged on an outer surface of the light filtering film (1); the holding housing (6) encloses the micro linear array image sensor chip (5), the light filtering film (1) and the parallel light source (4);
transmitted light generated by the parallel light source (4) passes through a transparent glass tube of the injection needle (c); then the transmitted light passes through the light filtering film (1) to reduce the intensity of the parallel light source (4) to a photosensitivity range of the micro linear array image sensor chip (5); finally, the transmitted light enters the micro linear array image sensor chip (5), so that the micro linear array image sensor chip (5) measures the change of the liquid level in the injection needle (c).