CPC C07K 14/55 (2013.01) [C07K 14/7155 (2013.01); A61K 38/00 (2013.01); C07K 2319/00 (2013.01)] | 1 Claim |
1. A modified human CD25 polypeptide engineered to have increased affinity for IL-2, relative to native CD25 protein and affinity of the modified CD25 protein for its cognate IL-2 protein is less than 0.5 nM, comprising a set of amino acid modifications selected from the group consisting of:
(1) D4E, M25A, N27V, E29D, S39Δ, G40Δ, S41T, L42A, I118T, H120L, and K153E;
(2) M25I, N27V, E29D, L42A, I118R, H120W, and K153Q;
(3) L2Q, M25I, N27Y, L42A, I118R, H120W, and K153G;
(4) L2Q, M25V, N27Y, L42A, I118R, and H120W; and
(5) M25L, N27V, L42A, I118N, H120M, and K153G.
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