US 11,780,891 B2
Insecticidal proteins and methods for their use
Steven D Gruver, Pacifica, CA (US); Heather Kozy, Walnut Creek, CA (US); Jessica O'Rear, Redwood City, CA (US); Barbara Rosen, Mountain View, CA (US); Ute Schellenberger, Palo Alto, CA (US); Jun-Zhi Wei, Johnston, IA (US); Weiping Xie, East Palo Alto, CA (US); Xiaohong Zhong, San Leandro, CA (US); and Genhai Zhu, San Jose, CA (US)
Assigned to PIONEER HI-BRED INTERNATIONAL, INC.
Filed by PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
Filed on Nov. 3, 2021, as Appl. No. 17/453,439.
Application 17/453,439 is a continuation of application No. 17/034,019, filed on Sep. 28, 2020, granted, now 11,203,619.
Application 17/034,019 is a continuation of application No. 16/567,200, filed on Sep. 11, 2019, granted, now 10,820,596, issued on Nov. 3, 2020.
Application 16/567,200 is a continuation of application No. 16/259,270, filed on Jan. 28, 2019, granted, now 10,448,648, issued on Oct. 22, 2019.
Application 16/259,270 is a continuation of application No. 15/543,689, granted, now 10,231,460, issued on Mar. 19, 2019, previously published as PCT/US2016/012473, filed on Jan. 7, 2016.
Claims priority of provisional application 62/103,787, filed on Jan. 15, 2015.
Prior Publication US 2022/0056082 A1, Feb. 24, 2022
Int. Cl. C07K 14/00 (2006.01); C07K 14/21 (2006.01); C12N 15/82 (2006.01); A01N 37/46 (2006.01); A01N 63/50 (2020.01)
CPC C07K 14/21 (2013.01) [A01N 37/46 (2013.01); A01N 63/50 (2020.01); C12N 15/8286 (2013.01); Y02A 40/146 (2018.01)] 9 Claims
 
1. An insecticidal polypeptide selected from:
a) a PIP-74-1 polypeptide comprising an amino acid sequence having greater than 75% sequence identity compared to SEQ ID NO: 73; or
b) a PIP-74-1 polypeptide comprising the amino acid sequence of SEQ ID NO: 73, SEQ ID NO: 75, or SEQ ID NO: 77; and
c) a PIP-74-2 polypeptide comprising an amino acid sequence having greater than 80% sequence identity compared to the amino acid sequence of SEQ ID NO: 74; or
d) a PIP-74-2 polypeptide comprising the amino acid sequence of SEQ ID NO: 74, SEQ ID NO: 76 or SEQ ID NO: 78;
wherein the insecticidal polypeptide is operably linked to a heterologous signal peptide or transit peptide, and wherein the PIP-74-1 polypeptide of a) or b) and the PIP-74-2 polypeptide of c) or d) in combination have insecticidal activity against Western corn rootworm.