	US 11,780,885 B2
	Zika viral antigen constructs
Kimberly Dowd, Rockville, MD (US); Barney S. Graham, Rockville, MD (US); Sung-Youl Ko, Rockville, MD (US); Wing-Pui Kong, Rockville, MD (US); John Mascola, Rockville, MD (US); Theodore Pierson, Rockville, MD (US); Mayuri Sharma, Cambridge, MA (US); and Dong Yu, Rockville, MD (US)
Assigned to GLAXOSMITHKLINE BIOLOGICALS SA, Rixensart (BE); and THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES, Bethesda, MD (US)
Appl. No. 16/461,503
Filed by GLAXOSMITHKLINE BIOLOGICALS SA, Rixensart (BE); and THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES, Bethesda, MD (US)
PCT Filed Nov. 15, 2017, PCT No. PCT/EP2017/079343 § 371(c)(1), (2) Date May 16, 2019, PCT Pub. No. WO2018/091540, PCT Pub. Date May 24, 2018.
Claims priority of provisional application 62/568,559, filed on Oct. 5, 2017.
Claims priority of provisional application 62/485,090, filed on Apr. 13, 2017.
Claims priority of provisional application 62/423,398, filed on Nov. 17, 2016.
Prior Publication US 2019/0345205 A1, Nov. 14, 2019
Int. Cl. C07K 14/005 (2006.01); A61K 39/12 (2006.01); C12N 7/00 (2006.01); A61K 39/00 (2006.01)

CPC C07K 14/005 (2013.01) [A61K 39/12 (2013.01); C12N 7/00 (2013.01); A61K 2039/53 (2013.01); C12N 2770/24122 (2013.01); C12N 2770/24134 (2013.01)]

19 Claims

1. A self-replicating RNA molecule encoding (i) a polypeptide comprising a Zika virus prME antigen which comprises amino acids 21 through 692 of SEQ ID NO: 2 or a polypeptide which comprises a region that is at least 90% identical to the amino sequence of SEQ ID NO:2 or an immunogenic fragment thereof comprising a contiguous amino acid sequence of at least 8 amino acids which is identical to a contiguous amino acids sequence of the full-length Zika virus prME antigen and (ii) a Japanese Encephalitis Virus (JEV) signal sequence comprising SEQ ID NO:5 or a sequence with at least 90% sequence identity to SEQ ID NO:5.