CPC A01H 5/12 (2013.01) [A01H 6/823 (2018.05); A24B 13/00 (2013.01); A24B 15/10 (2013.01); C12N 9/0073 (2013.01); C12N 9/0077 (2013.01); C12N 15/8243 (2013.01); C12Q 1/6895 (2013.01); C12Q 2600/13 (2013.01); C12Q 2600/156 (2013.01)] | 16 Claims |
1. A method for reducing the level of nornicotine or N′-nitrosonornicotine in a Nicotiana tabacum plant, the method comprising generating a stable mutation in a nicotine demethylase in the Nicotiana tabacum plant, wherein the stable mutation inhibits the expression or function of the nicotine demethylase, wherein the nicotine demethylase is a polypeptide comprising an amino acid sequence at least 98% identical to SEQ ID NO: 4, and wherein the amount of nornicotine or N′-nitrosonornicotine is reduced by greater than about 10% compared to a control Nicotiana tabacum plant comprising a wild-type allele of the nicotine demethylase.
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