US RE50,621 E1
Detecting breast cancer
David A. Ahlquist, Rochester, MN (US); William R. Taylor, Lake City, MN (US); Douglas W. Mahoney, Elgin, MN (US); Tracy C. Yab, Rochester, MN (US); John B. Kisiel, Rochester, MN (US); Hatim T. Allawi, Middleton, WI (US); Graham P. Lidgard, Middleton, WI (US); and Michael W. Kaiser, Stoughton, WI (US)
Assigned to Mayo Foundation for Medical Education and Research, Rochester, MN (US); and Exact Sciences Corporation, Madison, WI (US)
Filed by Mayo Foundation for Medical Education and Research, Rochester, MN (US); and Exact Sciences Corporation, Madison, WI (US)
Filed on May 3, 2022, as Appl. No. 17/735,477.
Application 17/735,477 is a reissue of application No. 16/202,944, filed on Nov. 28, 2018, granted, now 10,934,594, issued on Mar. 2, 2021.
Claims priority of provisional application 62/592,828, filed on Nov. 30, 2017.
Int. Cl. C12Q 1/68 (2018.01); C07H 21/04 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6809 (2018.01); C12Q 1/6811 (2018.01); C12Q 1/682 (2018.01); C12Q 1/6827 (2018.01); C12Q 1/6837 (2018.01); C12Q 1/6853 (2018.01); C12Q 1/6886 (2018.01); G16H 50/30 (2018.01)
CPC C12Q 1/6886 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6809 (2013.01); C12Q 1/6811 (2013.01); C12Q 1/682 (2013.01); C12Q 1/6827 (2013.01); C12Q 1/6837 (2013.01); C12Q 1/6853 (2013.01); G16H 50/30 (2018.01)] 10 Claims
 
1. A method, comprising:
measuring a methylation level for four genes in a biological sample of a human individual through
treating genomic DNA in the biological sample with a reagent that modifies DNA in a methylation-specific manner;
amplifying the treated genomic DNA using a specific set of primers for each of the selected genes; and
determining the methylation level of the four genes by polymerase chain reaction, nucleic acid sequencing, mass spectrometry, methylation-specific nuclease, mass-based separation, and [ or ] target capture;
wherein the four genes are TRIM67, OTX1, MPZ and BANK1.