US 12,436,498 B1
System and method for capturing and analyzing microscale and nanoscale digital holograms
Eduardo A. Sciammarella, Fallbrook, CA (US); Cesar A. Sciammarella, Chicago, IL (US); and Luciano Lamberti, Bari (IT)
Filed by Eduardo A. Sciammarella, Fallbrook, CA (US); Cesar A. Sciammarella, Chicago, IL (US); and Luciano Lamberti, Bari (IT)
Filed on Nov. 4, 2024, as Appl. No. 18/936,292.
Claims priority of provisional application 63/546,942, filed on Nov. 2, 2023.
Int. Cl. G03H 1/00 (2006.01); G02B 21/26 (2006.01); G02B 21/36 (2006.01); G03H 1/04 (2006.01)
CPC G03H 1/0005 (2013.01) [G02B 21/26 (2013.01); G02B 21/365 (2013.01); G03H 1/0443 (2013.01); G03H 2001/005 (2013.01); G03H 2210/55 (2013.01); G03H 2222/12 (2013.01); G03H 2222/31 (2013.01); G03H 2223/23 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A digital holographic optical microscope system for nanoscale imaging, comprises:
a microscope;
a camera sensor coupled to the microscope and positioned to capture images of objects within the field of view of an objective on the microscope;
a first optical grating positioned within the field of view of the microscope;
a microscope slide holding a sample specimen adjacent the first optical grating, wherein the microscope slide and the first optical grating meet at a first interface;
a second optical grating positioned on a side of the microscope slide opposite the first optical grating, wherein the microscope slide and the second optical grating meet at a second interface;
a light source positioned to illuminate the field of view of the microscope;
a first pumped laser and polarized output pointed at the first interface, wherein a critical angle of incidence of the first pumped laser and polarized output on the first interface is greater than a critical angle of the first interface;
a second pumped laser and polarized output pointed at the second interface, wherein a critical angle of incidence of the second pumped laser and polarized output on the second interface is greater than a critical angle of the second interface;
a motorized stage supporting the first optical grating, the microscope slide, and the second optical grating; and
an interface control system coupled to the motorized stage and the camera sensor, including a computing device configured to:
control a movement of the motorized stage to position the microscope slide and sample specimen within a plurality of adjusted positions within the field of view of the microscope;
receive image data from one or more evanescent wavefronts in images captured by the camera sensor;
interpret the image data using a Moiré holographic analysis;
reconstruct a three-dimensional image of the interpreted image data;
run a prediction engine on the three-dimensional image; and
identify features in the sample specimen using the prediction engine.