US 12,436,153 B2
Methods, compositions, and kits for high-throughput release, labeling, and analysis of O-glycans present on a glycoconjugate
Ace Gita Galermo, Greenfield, CA (US)
Assigned to Agilent Technologies, Inc., Santa Clara, CA (US)
Appl. No. 17/781,283
Filed by Agilent Technologies, Inc., Santa Clara, CA (US)
PCT Filed Dec. 10, 2020, PCT No. PCT/US2020/064337
§ 371(c)(1), (2) Date May 31, 2022,
PCT Pub. No. WO2021/119333, PCT Pub. Date Jun. 17, 2021.
Claims priority of provisional application 62/948,068, filed on Dec. 13, 2019.
Prior Publication US 2022/0412985 A1, Dec. 29, 2022
Int. Cl. B01L 3/00 (2006.01); B01D 15/08 (2006.01); B01F 33/302 (2022.01); B01F 33/3033 (2022.01); B01J 20/285 (2006.01); B01J 20/287 (2006.01); B01J 20/32 (2006.01); B01L 7/00 (2006.01); B01L 9/00 (2006.01); B65G 47/80 (2006.01); B82Y 20/00 (2011.01); B82Y 30/00 (2011.01); B82Y 40/00 (2011.01); C12M 1/34 (2006.01); C12M 3/06 (2006.01); C12N 1/14 (2006.01); C12N 1/20 (2006.01); C12Q 1/02 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/6848 (2018.01); C12Q 1/686 (2018.01); G01N 15/10 (2024.01); G01N 15/14 (2024.01); G01N 15/1433 (2024.01); G01N 21/29 (2006.01); G01N 21/65 (2006.01); G01N 30/02 (2006.01); G01N 30/60 (2006.01); G01N 30/72 (2006.01); G01N 33/543 (2006.01); G01N 33/557 (2006.01); G01N 33/574 (2006.01); G01N 33/58 (2006.01); G01N 33/68 (2006.01)
CPC G01N 33/6842 (2013.01) [G01N 33/58 (2013.01); G01N 2400/12 (2013.01); G01N 2440/38 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for releasing, labeling, and, optionally, analyzing, O-glycans present on a selected glycoconjugate of interest, said method comprising: incubating in a container said selected glycoconjugate of interest in an aqueous solution comprising 1.20M-2M triethylamine (“TEA”), and 0.20M to 1.25M 1-phenyl-3-methyl-5-pyrazolone (“PMP”) at a temperature of 70-100° C. for a time between about 10 minutes and 23 hours.