US 12,436,152 B2
Methods for predicting and modulating glycation of a protein
Haibo Qiu, Millwood, NY (US); Xiaobin Xu, Old Greenwich, CT (US); Douglas Kamen, Poughquag, NY (US); Teng-Chieh Yang, Stratford, CT (US); and Kenneth Graham, Pleasant Valley, NY (US)
Assigned to Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed by Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed on Apr. 1, 2022, as Appl. No. 17/711,817.
Claims priority of provisional application 63/170,330, filed on Apr. 2, 2021.
Prior Publication US 2022/0326250 A1, Oct. 13, 2022
Int. Cl. G01N 33/68 (2006.01); A61K 39/00 (2006.01); C07K 16/28 (2006.01)
CPC G01N 33/6803 (2013.01) [C07K 16/28 (2013.01); A61K 2039/505 (2013.01); A61K 2039/545 (2013.01); C07K 2317/24 (2013.01); C07K 2317/52 (2013.01); C07K 2317/565 (2013.01); C07K 2317/92 (2013.01); C07K 2317/94 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method for maintaining a glycation percentage of an amino acid in a biomolecule within a predetermined glycation percentage range over a shelf-life of the biomolecule, comprising:
incubating the biomolecule having a first glycation percentage in a plurality of different glucose concentrations, and measuring the glycation percentage over time for each of the plurality of different glucose concentrations;
determining a glycation equilibrium percentage for the biomolecule as a function of each of the plurality of different glucose concentrations based on the measuring of the glycation percentage over time;
identifying the glycation equilibrium percentage and a corresponding glucose concentration that results in the first glycation percentage being maintained within the predetermined glycation percentage range; and
incubating the biomolecule with the corresponding glucose concentration over the shelf-life of the biomolecule.